endogenous biotin (IBA Lifesciences)

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IBA Lifesciences endogenous biotin
Endogenous Biotin, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endogenous+biotin/Biotin/bio_rxiv__2025__10__06__680766-350-12-23
Average 96 stars, based on 700 article reviews

endogenous biotin - by Bioz Stars, 2026-09

96/100 stars

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Centrifugation:

Article Title: A new structural paradigm for outer membrane protein biogenesis in the Bacteroidota
Article Snippet: .. The supernatant was recovered and total membranes were collected by centrifugation at 230,000 g for 75 min. Membranes were resuspended in buffer W to a protein concentration of 6.5 mg/ml and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) for 2 h. Insoluble material was removed by centrifugation at 230,000 g for 75 min. Endogenous biotin-containing proteins were masked by addition of 1 ml BioLock solution (IBA Lifesciences) per 100 ml of supernatant and incubation for 20 min with constant stirring. .. The solution was then circulated through a Strep-TactinXT 4Flow High Capacity column (IBA Lifesciences) overnight.

Article Title: A new paradigm for outer membrane protein biogenesis in the Bacteroidota.
Article Snippet: .. The supernatant was recovered and total membranes were collected by centrifugation at 230,000g for 75 min. Membranes were resuspended in buffer W to a protein concentration of 6.5 mg ml−1 and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) for 2 h. Insoluble material was removed by centrifugation at 230,000g for 75 min. Endogenous biotin-containing proteins were masked by addition of 1 ml BioLock solution (IBA Lifesciences) per 100 ml of supernatant and incubation for 20 min with constant stirring. .. The solution was then circulated through a Strep-TactinXT 4Flow High Capacity column (IBA Lifesciences) overnight.

Article Title: Structural characterization of protein transport by the Type 9 Secretion System
Article Snippet: .. The supernatant was recovered and total membranes were collected by centrifugation at 230,000g for 75 min. Membranes were resuspended in buffer W to a protein concentration of 6.5 mg/ml and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) for 2 h. Insoluble material was removed by centrifugation at 230,000g for 75 min. Endogenous biotin-containing proteins were masked by addition of 1 ml BioLock (IBA Lifesciences) solution per 100 ml of supernatant and incubation for 20 min with constant stirring. .. The solution was then circulated through a Strep-TactinXT Superflow column (IBA Lifesciences) overnight.

Article Title: Structural insights into the mechanism of protein transport by the Type 9 Secretion System translocon
Article Snippet: .. The supernatant was recovered and total membranes were collected by centrifugation at 230,000 g for 75 min. Membranes were resuspended in buffer W to a protein concentration of 6.5 mg ml −1 and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) for 2 h. Insoluble material was removed by centrifugation at 230,000 g for 75 min. Endogenous biotin-containing proteins were masked by addition of 1 ml BioLock (IBA Lifesciences) solution per 100 ml of supernatant and incubation for 20 min with constant stirring. .. The solution was then circulated through a Strep-TactinXT Superflow column (IBA Lifesciences) overnight.

Article Title: A new paradigm for outer membrane protein biogenesis in the Bacteroidota
Article Snippet: .. The supernatant was recovered and total membranes were collected by centrifugation at 230,000 g for 75 min. Membranes were resuspended in buffer W to a protein concentration of 6.5 mg ml −1 and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) for 2 h. Insoluble material was removed by centrifugation at 230,000 g for 75 min. Endogenous biotin-containing proteins were masked by addition of 1 ml BioLock solution (IBA Lifesciences) per 100 ml of supernatant and incubation for 20 min with constant stirring. .. The solution was then circulated through a Strep-TactinXT 4Flow High Capacity column (IBA Lifesciences) overnight.

Protein Concentration:

Incubation:

Article Title: Regulation of the formin INF2 by actin monomers and calcium-calmodulin
Article Snippet: .. The supernatant was incubated with 20 μg/ml avidin (Sigma-Aldrich; 189725) to block endogenous biotin before loading onto a 5 ml Strep-Tactin Superflow column (IBA; 2-1206-025) pre-equilibrated with EB-S buser. ..

Article Title: Rapid discovery and classification of inhibitors of coronavirus infection by pseudovirus screen and amplified luminescence proximity homogeneous assay
Article Snippet: .. The supernatant containing RBD was equilibrated by the addition of 100 mL of 10× buffer W (1 M Tris-Cl, pH 8.0, 1.5 M NaCl, 10 mM EDTA), and endogenous biotin was blocked by incubation with 2.4 mL of BioLock Biotin blocking solution (IBA Lifesciences, Göttingen, Germany). .. Debris was filtered out using a NalgeneTM Rapid-FlowTM filter (Thermo Scientific).

Article Title: Rapid discovery and classification of inhibitors of coronavirus infection by pseudovirus screen and amplified luminescence proximity homogeneous assay.
Article Snippet: .. The supernatant containing RBD was equilibrated by the addition of 100 mL of 10× buffer W (1 M Tris-Cl, pH 8.0, 1.5 M NaCl, 10 mM EDTA), and endogenous biotin was blocked by incubation with 2.4 mL of BioLock Biotin blocking solution (IBA Lifesciences, Göttingen, Germany). .. Debris was filtered out using a NalgeneTM Rapid-FlowTM filter (Thermo Scientific).

Avidin-Biotin Assay:

Blocking Assay:

Article Title: Rapid discovery and classification of inhibitors of coronavirus infection by pseudovirus screen and amplified luminescence proximity homogeneous assay.
Article Snippet: .. The supernatant containing RBD was equilibrated by the addition of 100 mL of 10× buffer W (1 M Tris-Cl, pH 8.0, 1.5 M NaCl, 10 mM EDTA), and endogenous biotin was blocked by incubation with 2.4 mL of BioLock Biotin blocking solution (IBA Lifesciences, Göttingen, Germany). .. Debris was filtered out using a NalgeneTM Rapid-FlowTM filter (Thermo Scientific).

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